Meiotic spindle visualization in living human oocytes
نویسندگان
چکیده
منابع مشابه
O-11: Zona Pellucida Birefringence and Meiotic Spindle Visualization of Human Oocytes Are Not Influenced by In Vitro Maturation Technology
Background: In vitro maturation (IVM) is a promising treatment option for certain infertile women. Nowadays, with the aid of the Polscope, it has become possible to evaluate zona pellucida (ZP) and spindle as parameters of oocyte quality. The goal was to investigate the relationship between the presence of the meiotic spindle and ZP birefringence with morphology of the in vivo and in vitro matu...
متن کاملP-120: Meiotic Spindle Visualization and Zona Pellucida Birefringence in Relation to Morphology of In Vivo and In Vitro Matured Human Oocytes of In Vivo and In Vitro Matured Human Oocytesof In Vivo and In Vitro Matured Human Oocytes
Background: The meiotic spindle plays an important role in the oocyte during chromosome alignment and separation at meiosis. The zona pellucid (ZP) is a dynamic matrix composed of filaments with the properties that might reflect the history of oocyte cytoplasmic maturation. Since, spindle and ZP in living oocytes are highly birefringent, their structures can be viewed noninvasively by using a P...
متن کاملRelationship between visualization of meiotic spindle in human oocytes and ICSI outcomes: a meta-analysis.
The objective of this meta-analysis was to investigate the influence of meiotic spindle visualization in human oocytes on intracytoplasmic sperm injection (ICSI) outcomes. Search strategies included on-line surveys of databases (MEDLINE, EMBASE, Science Citation Index, Cochrane Controlled Trials Register and Ovid). The fixed effect was used for odds ratio. Ten trials fulfilled the inclusion cri...
متن کاملThe Influence of Meiotic Spindle Configuration by Cysteamine during in vitro Maturation of Mouse Oocytes
Background: The aim of this study was to assess effects of cysteamine as an anti-oxidant on rate of in vitro maturation of oocyte and determination of its effects on spindle size and shape. Methods: Pre-mature mice were primed with pregnant mare stimulating gonadotrophin (PMSG) and germinal vesicle (GV) stage oocytes were obtained 48 h after. The oocytes were cultured in tissue culture medium (...
متن کاملPolscope analysis of meiotic spindle changes in living metaphase II human oocytes during the freezing and thawing procedures.
BACKGROUND The clinical efficacy of the current methods used for cryopreservation of metaphase II human oocytes is low. Meiotic spindle disorders are thought to be largely responsible for this situation. METHODS Supernumerary fresh metaphase II human oocytes were cryopreserved in 1,2-propanediol with 0.1 M sucrose using a slow freezing/rapid thawing programme. Meiotic spindles were analysed i...
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ژورنال
عنوان ژورنال: Reproductive BioMedicine Online
سال: 2005
ISSN: 1472-6483
DOI: 10.1016/s1472-6483(10)60940-6